PMID:8798391
| Citation |
Guillon, JM, Heiss, S, Soutourina, J, Mechulam, Y, Laalami, S, Grunberg-Manago, M and Blanquet, S (1996) Interplay of methionine tRNAs with translation elongation factor Tu and translation initiation factor 2 in Escherichia coli. J. Biol. Chem. 271:22321-5 |
|---|---|
| Abstract |
According to their role in translation, tRNAs specifically interact either with elongation factor Tu (EFTu) or with initiation factor 2 (IF2). We here describe the effects of overproducing EFTu and IF2 on the elongator versus initiator activities of various mutant tRNAMet species in vivo. The data obtained indicate that the selection of a tRNA through one or the other pathway of translation depends on the relative amounts of the translational factors. A moderate overexpression of EFTu is enough to lead to a misappropriation of initiator tRNA in the elongation process, whereas overproduced IF2 allows the initiation of translation to occur with unformylated tRNA species. In addition, we report that a strain devoid of formylase activity can be cured by the overproduction of tRNAMetf. The present study brings additional evidence for the importance of formylation in defining tRNAMetf initiator identity, as well as a possible explanation for the residual growth of bacterial strains lacking a functional formylase gene such as observed in Guillon, J. M., Mechulam, Y., Schmitter, J.-M., Blanquet, S., and Fayat, G. (1992) J. Bacteriol. 174, 4294-4301. |
| Links | |
| Keywords |
Cloning, Molecular; Cosmids; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Amplification; Peptide Elongation Factor Tu/metabolism; Peptide Initiation Factors/metabolism; Plasmids/metabolism; Prokaryotic Initiation Factor-2; Protein Biosynthesis; RNA, Transfer, Met/metabolism; Restriction Mapping |
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