PMID:8761731

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Citation

Du, Y, Holtel, A, Reizer, J and Saier, MH Jr sigma54-dependent transcription of the Pseudomonas putida xylS operon is influenced by the IIANtr protein of the phosphotransferase system in Escherichia coli. Res. Microbiol. 147:129-32

Abstract

IIANtr, encoded within the rpoN operons of many Gram-negative bacteria, is a homologue of a class of phosphoryl transfer proteins of the phosphoenolpyruvate: sugar phosphotransferase system. We have used a xylS operon-lacZ fusion from the TOL plasmid of Pseudomonas putida to show that IIANtr influences sigma 54-dependent transcription when the xylS operon is expressed in Escherichia coli. Loss of IIANtr influences, but does not abolish cyclic AMP-independent carbon catabolite repression.

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Keywords

Bacterial Proteins/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Operon/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Pseudomonas putida/genetics; Sigma Factor/genetics; Transcription, Genetic

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