PMID:8757730
| Citation |
Yang, M, Luoh, SM, Goddard, A, Reilly, D, Henzel, W and Bass, S (1996) The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase. Microbiology (Reading, Engl.) 142 ( Pt 7):1659-65 |
|---|---|
| Abstract |
A new Escherichia coli gene, bgIX, encoding a beta-D-glucosidase (EC 3.2.1.21) has been characterized. The bgIX gene is located adjacent to the dld gene at 47.8 min or 2225 kb on the E. coli chromosome. The sequence of a 2.6 kb DNA fragment from this region revealed a large open reading frame encoding a protein of 765 amino acids. The BgIX protein was purified from the periplasm, and amino-terminal sequencing suggests that the mature protein is derived from cleavage of a 20 residue signal peptide. A search of the sequence databases revealed that BgIX is a member of a large family of beta-glucosidases from a variety of bacteria and fungi, and the plant Arabidopsis thaliana. It differs from the other four E. coli phospho-beta-glucosidases in sequence and in its periplasmic rather than cytoplasmic location. The BgIX enzyme has a Km of 18 +/- 1 mM and a Vmax of 3 +/- 0.7 mumol min-1 for the colorimetric substrate o-nitrophenyl beta-D-glucopyranoside. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/genetics; Cloning, Molecular; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Phenotype; Sequence Homology, Amino Acid; beta-Glucosidase/genetics |
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