PMID:8755556
| Citation |
Ochman, H, Soncini, FC, Solomon, F and Groisman, EA (1996) Identification of a pathogenicity island required for Salmonella survival in host cells. Proc. Natl. Acad. Sci. U.S.A. 93:7800-4 |
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| Abstract |
We have identified a region unique to the Salmonella typhimurium chromosome that is essential for virulence in mice. This region harbors at least three genes: two (spiA and spiB) encode products that are similar to proteins found in type III secretion systems, and a third (spiR) encodes a putative regulator. A strain with a mutation in spiA was unable to survive within macrophages but displayed wild-type levels of epithelial cell invasion. The culture supernatants of the spi mutants lacked a modified form of flagellin, which was present in the supernatant of the wild-type strain. This suggests that the Spi secretory apparatus exports a protease, or a protein that can alter the activity of a secreted protease. The "pathogenicity island" harboring the spi genes may encode the virulence determinants that set Salmonella apart from other enteric pathogens. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Cell Line; Cell Survival; Endopeptidases/metabolism; Flagellin/genetics; Genes, Bacterial; Macrophages/cytology; Macrophages/microbiology; Mice; Molecular Sequence Data; Mutation; Salmonella typhimurium/genetics; Salmonella typhimurium/growth & development; Salmonella typhimurium/pathogenicity; Sequence Homology, Amino Acid; Virulence/genetics |
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