PMID:8746842
| Citation |
Jensen, PR, Michelsen, O and Westerhoff, HV (1995) Experimental determination of control by the H(+)-ATPase in Escherichia coli. J. Bioenerg. Biomembr. 27:543-54 |
|---|---|
| Abstract |
Strains carrying deletions in the atp genes, encoding the H(+)-ATPase, were unable to grow on nonfermentable substrates such as succinate, whereas with glucose as the substrate the growth rate of an atp deletion mutant was surprisingly high (some 75-80% of wild-type growth rate). The rate of glucose and oxygen consumption of these mutants was increased compared to the wild-type rates. In order to analyze the importance of the H(+)-ATPase at its physiological level, the cellular concentration of H(+)-ATPase was modulated around the wild-type level, using genetically manipulated strains. The control coefficient by the H(+)-ATPase with respect to growth rate and catabolic fluxes was measured. Control on growth rate was absent at the wild-type concentration of H(+)-ATPase, independent of whether the substrate for growth was glucose or succinate. Control by the H(+)-ATPase on the catabolic fluxes, including respiration, was negative at the wild-type H(+)-ATPase level. Moreover, the turnover number of the individual H(+)-ATPase enzymes increased as the H(+)-ATPase concentration was lowered. The negative control by the H(+)-ATPase on catabolism may thus be involved in a homeostatic control of ATP synthesis and, to some extent, explain the zero control by the H(+)-ATPase on E. coli growth rate. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Gene Deletion; Genes, Bacterial; Glucose/metabolism; Kinetics; Operon; Oxygen Consumption; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/metabolism; Succinates/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.