PMID:8730873
| Citation |
Oberto, J, Bonnefoy, E, Mouray, E, Pellegrini, O, Wikström, PM and Rouvière-Yaniv, J (1996) The Escherichia coli ribosomal protein S16 is an endonuclease. Mol. Microbiol. 19:1319-30 |
|---|---|
| Abstract |
The histone-like protein HU isolated from Escherichia coli exhibited, after several purification steps, a Mg(2+)-dependent nuclease activity. We show here that this activity can be dissociated from HU by a denaturation-renaturation step, and is due to a small fraction of ribosomal protein S16 co-purifying with HU. S16 is an essential component of the 30S ribosomal particles. We have cloned, overproduced, and purified a histidine-tagged S16 and shown that this protein is a DNA-binding protein carrying a Mg(2+)-Mn(2+)-dependent endonuclease activity. This is an unexpected property for a ribosomal protein. |
| Links | |
| Keywords |
Bacterial Proteins/isolation & purification; Base Sequence; DNA Primers/genetics; DNA, Bacterial/genetics; DNA-Binding Proteins/isolation & purification; Endonucleases/genetics; Endonucleases/isolation & purification; Endonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Magnesium/metabolism; Manganese/metabolism; Molecular Sequence Data; Ribosomal Proteins/genetics; Ribosomal Proteins/isolation & purification; Ribosomal Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
