PMID:8721992
| Citation |
Frank, J, Verschoor, A, Li, Y, Zhu, J, Lata, RK, Radermacher, M, Penczek, P, Grassucci, R, Agrawal, RK and Srivastava, S A model of the translational apparatus based on a three-dimensional reconstruction of the Escherichia coli ribosome. Biochem. Cell Biol. 73:757-65 |
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| Abstract |
The morphology of the Escherichia coli ribosome, i.e., its shape at moderate to low (20-40 A (1 A = 0.1 nm)) resolution, provides important constraints in modeling both the folding of ribosomal RNA and the translational process. A new reconstruction, obtained by low-dose cryoelectron microscopy and image processing of single ribosomes, contains clues to the way in which the ribosome interacts with the key functional ligands: the mRNA and the A- and P-site tRNAs. It also suggests possible pathways of the nascent polypeptide chain. From an interpretation of these clues in the light of existing knowledge, a plausible model for the locations and interactions of key components of protein synthesis is suggested. |
| Links | |
| Keywords |
Escherichia coli/genetics; Escherichia coli/ultrastructure; Image Processing, Computer-Assisted; Microscopy, Electron; Models, Genetic; Protein Biosynthesis; RNA, Bacterial/genetics; Ribosomes/ultrastructure |
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