PMID:8692841
| Citation |
Reardon, JT, Ge, H, Gibbs, E, Sancar, A, Hurwitz, J and Pan, ZQ (1996) Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH. Proc. Natl. Acad. Sci. U.S.A. 93:6482-7 |
|---|---|
| Abstract |
Transcription factor IIH (TFIIH) is a multisubunit protein complex essential for both the initiation of RNA polymerase class II (pol II)-catalyzed transcription and nucleotide excision repair of DNA. Recent studies have shown that TFIIH copurifies with the cyclin-dependent kinase (cdk)-activating kinase complex (CAK) that includes cdk7, cyclin H, and p36/MAT1. Here we report the isolation of two TFIIH-related complexes: TFIIH* and ERCC2/CAK. TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK. ERCC2/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36/MAT1) as well as the ERCC2 (XPD) protein. TFIIH* can support pol II-catalyzed transcription in vitro with lower efficiency compared with TFIIH. This TFIIH*-dependent transcription reaction was stimulated by ERCC2/CAK. The ERCC2/CAK and TFIIH* complexes are each active in DNA repair as shown by their ability to complement extracts prepared from ERCC2 (XPD)- and ERCC3 (XPB)-deficient cells, respectively, in supporting the excision of DNA containing a cholesterol lesion. These data suggest that TFIIH* and ERCC2/CAK interact to form the TFIIH holoenzyme capable of efficiently assembling the pol II transcription initiation complex and directly participating in excision repair reactions. |
| Links | |
| Keywords |
Animals; CHO Cells; Chromatography, Ion Exchange; Cricetinae; Cyclin-Dependent Kinases/isolation & purification; Cyclin-Dependent Kinases/metabolism; DNA Helicases; DNA Repair; DNA-Binding Proteins; Humans; Proteins/isolation & purification; Proteins/metabolism; RNA Polymerase II/metabolism; Transcription Factor TFIIH; Transcription Factors/isolation & purification; Transcription Factors/metabolism; Transcription Factors, TFII; Transcription, Genetic; Xeroderma Pigmentosum Group D Protein |
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