PMID:8682785
| Citation |
Alm, RA and Mattick, JS (1996) Identification of two genes with prepilin-like leader sequences involved in type 4 fimbrial biogenesis in Pseudomonas aeruginosa. J. Bacteriol. 178:3809-17 |
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| Abstract |
Type 4 fimbriae are surface filaments produced by a range of bacterial pathogens for colonization of host epithelial surfaces. In Pseudomonas aeruginosa, they are involved in adhesion as well as in a form of surface translocation called twitching motility, and sensitivity to infection by fimbria-specific bacteriophage. Analysis of the 2.5-kb intergenic region between the previously defined pilR and pilV genes on P. aeruginosa genomic SpeI fragment E has identified three new genes, fimT, fimU, and dadA*. The predicted 18.5-kDa products of the fimT and fimU genes contain prepilin-like leader sequences, whereas the third gene, dadA*, encodes a protein similar to the D-amino acid dehydrogenase of Escherichia coli. Isogenic mutants constructed by allelic exchange demonstrated that the fimU gene was required for fimbrial biogenesis and twitching motility, whereas the fimT and dada* mutants retained wild-type phenotypes. However, overexpression of the fimT gene was found to be able to functionally replace the lack of a fimU gene product, suggesting a subtle role in fimbrial biogenesis. The identification of these proteins increases the similarity between type 4 fimbrial biogenesis and the supersystems involved in macromolecular traffic, such as extracellular protein secretion and DNA uptake, all of which now possess multiple protein species that possess prepilin-like leader sequences. |
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| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DNA, Bacterial; Fimbriae Proteins; Fimbriae, Bacterial/genetics; Gene Expression; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Mutation; Phenotype; Protein Precursors/genetics; Protein Sorting Signals/genetics; Pseudomonas aeruginosa/genetics; Sequence Analysis; Sequence Homology, Amino Acid; Transcription Factors/genetics |
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