PMID:8645235
| Citation |
Schwender, J, Seemann, M, Lichtenthaler, HK and Rohmer, M (1996) Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus. Biochem. J. 316 ( Pt 1):73-80 |
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| Abstract |
Isoprenoid biosynthesis was investigated in the green alga Scenedesmus obliquus grown heterotrophically on 13C-labelled glucose and acetate. Several isoprenoid compounds were isolated and investigated by 13C-NMR spectroscopy. According to the 13C-labelling pattern indicated by the 13C-NMR spectra, the biosynthesis of all plastidic isoprenoids investigated (prenyl side-chains of chlorophylls and plastoquinone-9, and the carotenoids beta-carotene and lutein), as well as of the non-plastidic cytoplasmic sterols, does not proceed via the classical acetate/mevalonate pathway (which leads from acetyl-CoA via mevalonate to isopentenyl diphosphate), but via the novel glyceraldehyde 3-phosphate/pyruvate route recently detected in eubacteria. Formation of isopentenyl diphosphate involves the condensation of a C2 unit derived from pyruvate decarboxylation with glyceraldehyde 3-phosphate and a transposition yielding the branched C5 skeleton of isoprenic units. |
| Links | |
| Keywords |
Carbon Isotopes; Carotenoids/biosynthesis; Chlorophyll/biosynthesis; Chlorophyta/metabolism; Glyceraldehyde 3-Phosphate/metabolism; Glyoxylates/metabolism; Magnetic Resonance Spectroscopy; Pentose Phosphate Pathway; Plastoquinone/metabolism; Pyruvates/metabolism; Sterols/biosynthesis; Terpenes/metabolism |
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