PMID:8643630
| Citation |
Tenson, T, DeBlasio, A and Mankin, A (1996) A functional peptide encoded in the Escherichia coli 23S rRNA. Proc. Natl. Acad. Sci. U.S.A. 93:5641-6 |
|---|---|
| Abstract |
A pentapeptide open reading frame equipped with a canonical ribosome-binding site is present in the Escherichia coli 23S rRNA. Overexpression of 23S rRNA fragments containing the mini-gene renders cells resistant to the ribosome-inhibiting antibiotic erythromycin. Mutations that change either the initiator or stop codons of the peptide mini-gene result in the loss of erythromycin resistance. Nonsense mutations in the mini-gene also abolish erythromycin resistance, which can be restored in the presence of the suppressor tRNA, thus proving that expression of the rRNA-encoded peptide is essential for the resistance phenotype. The ribosome appears to be the likely target of action of the rRNA-encoded pentapeptide, because in vitro translation of the peptide mini-gene decreases the inhibitory action of erythromycin on cell-free protein synthesis. Thus, the new mechanism of drug resistance reveals that in addition to the structural and functional role of rRNA in the ribosome, it may also have a peptide-coding function. |
| Links | |
| Keywords |
Amino Acid Sequence; Ampicillin Resistance; Base Sequence; Binding Sites; DNA Primers; DNA, Ribosomal/chemistry; DNA, Ribosomal/metabolism; Drug Resistance, Microbial; Erythromycin/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Suppressor; Molecular Sequence Data; Nucleic Acid Conformation; Open Reading Frames; Polymerase Chain Reaction; Protein Biosynthesis; RNA, Ribosomal, 23S/metabolism; Ribosomes/metabolism; Sequence Deletion |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.