PMID:8636054
| Citation |
Man, TK, Zhao, G and Winkler, ME (1996) Isolation of a pdxJ point mutation that bypasses the requirement for the PdxH oxidase in pyridoxal 5' -phosphate coenzyme biosynthesis in Escherichia coli K-12. J. Bacteriol. 178:2445-9 |
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| Abstract |
We isolated 26 suppressor mutations that allowed growth of a delta pdxH::omega null mutant in the absence of pyridoxal. Each suppressor mapped to pdxJ, and the eight suppressors sequenced contained the same glycine-to-serine change in the PdxJ polypeptide. This bypass suppression suggests that PdxJ may participate in formation of the pyridine ring of pyridoxine 5'-phosphate. |
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| Keywords |
Bacterial Proteins/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Ligases; Models, Biological; Mutagenesis, Insertional; Point Mutation; Pyridoxal Phosphate/analogs & derivatives; Pyridoxal Phosphate/biosynthesis; Pyridoxal Phosphate/metabolism; Pyridoxaminephosphate Oxidase/genetics; Pyridoxaminephosphate Oxidase/metabolism; Sequence Deletion; Suppression, Genetic |
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