PMID:8626309
| Citation |
Lazar, SW and Kolter, R (1996) SurA assists the folding of Escherichia coli outer membrane proteins. J. Bacteriol. 178:1770-3 |
|---|---|
| Abstract |
Many proteins require enzymatic assistance in order to achieve a functional conformation. One rate-limiting step in protein folding is the cis-trans isomerization of prolyl residues, a reaction catalyzed by prolyl isomerases. SurA, a periplasmic protein of Escherichia coli, has sequence similarity with the prolyl isomerase parvulin. We tested whether SurA was involved in folding periplasmic and outer membrane proteins by using trypsin sensitivity as an assay for protein conformation. We determined that the efficient folding of three outer membrane proteins (OmpA, OmpF, and LamB) requires SurA in vivo, while the folding of four periplasmic proteins was independent of SurA. We conclude that SurA assists in the folding of certain secreted proteins. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/drug effects; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carrier Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Heat-Shock Proteins; Molecular Sequence Data; Peptidylprolyl Isomerase; Periplasmic Proteins; Porins/metabolism; Protein Conformation; Protein Folding; Receptors, Virus/metabolism; Serine Endopeptidases/biosynthesis; Trypsin/pharmacology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
