PMID:8576210
| Citation |
Skaliter, R, Paz-Elizur, T and Livneh, Z (1996) A smaller form of the sliding clamp subunit of DNA polymerase III is induced by UV irradiation in Escherichia coli. J. Biol. Chem. 271:2478-81 |
|---|---|
| Abstract |
The beta subunit of DNA polymerase III holoenzyme of Escherichia coli is a 40.6-kDa protein that functions as a sliding DNA clamp (Stukenberg, P. T., Studwell-Vaughan, P. S., and O'Donnell, M. (1991) J. Biol. Chem. 266, 11328-11334). It is responsible for tethering the polymerase to DNA and endowing it with the high processivity required for DNA replication. Here and in a companion study (Paz-Elizur, T., Skaliter, R., Blumenstein, S., and Livneh, Z. (1996) J. Biol. Chem. 271, 2482-2490) we report that the dnaN gene, encoding the beta subunit, contains an internal in-frame gene, termed dnaN*, that encodes a smaller form of the beta subunit. The novel 26-kDa protein, termed beta*, is UV-inducible, and when overexpressed from a plasmid under an inducible promoter, it increases up to 6-fold the UV resistance of E. coli cells. These findings suggest that the beta* protein functions in a reaction associated with DNA repair or recovery of DNA replication in UV-irradiated cells. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; DNA Polymerase III/biosynthesis; DNA Polymerase III/genetics; DNA, Recombinant; Enzyme Induction; Escherichia coli/enzymology; Escherichia coli/radiation effects; Molecular Sequence Data; Plasmids; Ultraviolet Rays |
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