PMID:8566238
| Citation |
Laber, B, Clausen, T, Huber, R, Messerschmidt, A, Egner, U, Müller-Fahrnow, A and Pohlenz, HD (1996) Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase. FEBS Lett. 379:94-6 |
|---|---|
| Abstract |
The metC gene coding for cystathionine beta-lyase of Escherichia coli has been cloned and used to construct an overproducing E. coli strain. An efficient purification scheme has been developed and the purified enzyme has been crystallized by the hanging drop vapour diffusion method using either ammonium sulfate or polyethyleneglycol 400 as precipitating agent. The crystals belong to the orthorombic space group C222. Their unit cell parameters are a = 60.9 A, b = 154.7 A and c = 152.7 A. Consideration of the possible values of VM accounts for the presence of one dimer per asymmetric unit. The crystals are suitable for X-ray analysis and a complete native date set to 1.83 A resolution has been collected using synchrotron radiation. |
| Links | |
| Keywords |
Base Sequence; Cloning, Molecular; Crystallization; Crystallography, X-Ray; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Lyases/chemistry; Lyases/genetics; Lyases/isolation & purification; Molecular Sequence Data |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.