PMID:8526857
| Citation |
Smith, K, Borges, A, Ariyanayagam, MR and Fairlamb, AH (1995) Glutathionylspermidine metabolism in Escherichia coli. Biochem. J. 312 ( Pt 2):465-9 |
|---|---|
| Abstract |
Intracellular levels of glutathione and glutathionylspermidine conjugates have been measured throughout the growth phases of Escherichia coli. Glutathionylspermidine was present in mid-log-phase cells, and under stationary and anaerobic growth conditions accounted for 80% of the total glutathione content. N1,N8-bis(glutathionyl)spermidine (trypanothione) was undetectable under all growth conditions. The catalytic constant kcat/Km of recombinant E. coli glutathione reductase for glutathionylspermidine disulphide was approx. 11,000-fold lower than that for glutathione disulphide. The much higher catalytic constant for the mixed disulphide of glutathione and glutathionylspermidine (11% that of GSSG), suggests a possible explanation for the low turnover of trypanothione disulphide by E. coli glutathione reductase, given the apparent lack of a specific glutathionylspermidine disulphide reductase in E. coli. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; DNA Primers; Disulfides/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Glutathione/analogs & derivatives; Glutathione/metabolism; Glutathione Disulfide; Glutathione Reductase/biosynthesis; Glutathione Reductase/isolation & purification; Glutathione Reductase/metabolism; Kinetics; Models, Biological; Molecular Sequence Data; Oxidation-Reduction; Polymerase Chain Reaction; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Spermidine/analogs & derivatives; Spermidine/metabolism |
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