PMID:8526531
| Citation |
Kuhnert, P, Nicolet, J and Frey, J (1995) Rapid and accurate identification of Escherichia coli K-12 strains. Appl. Environ. Microbiol. 61:4135-9 |
|---|---|
| Abstract |
A specific PCR for the identification of K-12 strains, based on the genetic structure of the O-antigen gene cluster (rfb) of Escherichia coli K-12, is described. The assay clearly differentiates E. coli K-12-derived strains from other E. coli strains used in the laboratory or isolated from human and animal clinical specimens, from food, or from environmental samples. Moreover, lineages of K-12 strains can be distinguished with a second PCR based on the same gene cluster. The method presents a useful tool in identifying K-12 for monitoring strains which are used as biologically safe vehicles in biotechnological research, development, and production processes. |
| Links | |
| Keywords |
Animals; Base Sequence; Chromosome Mapping; DNA Primers/genetics; DNA, Bacterial/genetics; Environmental Microbiology; Environmental Monitoring; Escherichia coli/classification; Escherichia coli/genetics; Escherichia coli/isolation & purification; Food Microbiology; Genes, Bacterial; Humans; Molecular Sequence Data; Multigene Family; O Antigens; Phylogeny; Polymerase Chain Reaction/methods; Polymerase Chain Reaction/statistics & numerical data; Sensitivity and Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.