PMID:8508916
| Citation |
Price, S, Cusack, S, Borel, F, Berthet-Colominas, C and Leberman, R (1993) Crystallization of the seryl-tRNA synthetase:tRNAS(ser) complex of Escherichia coli. FEBS Lett. 324:167-70 |
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| Abstract |
Crystals of the complex between seryl-tRNA synthetase and tRNA(2ser) from Escherichia coli have been obtained from ammonium sulphate solutions. The crystals are of the 1:2 enzyme:tRNA complex, belong to the space group C222(1), have cell dimensions of a = 128.9 A, b = 164.9 A, c = 127.3 A and diffract anisotropically from 3.5 to 4.5 A. An X-ray diffraction data set to 4 A has been collected. The combination of molecular replacement using the refined structure of the catalytic domain of the native enzyme, data from a heavy atom derivative and solvent flattening was used to produce a map at 4 A resolution. This shows that a tRNA molecule binds across the dimer, the anticodon stem and loop do not contact the protein and the helical arm of the enzyme contacts the T psi C loop and the long extra arm of the tRNA. |
| Links | |
| Keywords |
Crystallization; Escherichia coli/chemistry; Macromolecular Substances; Models, Molecular; Protein Conformation; RNA, Transfer, Ser/chemistry; Serine-tRNA Ligase/chemistry; X-Ray Diffraction |
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