PMID:8496960
| Citation |
Weaver, TM, Levitt, DG and Banaszak, LJ (1993) Purification and crystallization of fumarase C from Escherichia coli. J. Mol. Biol. 231:141-4 |
|---|---|
| Abstract |
Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C222(1) form is complete to 2.10 A and the I222 form is complete to 2.20 A. |
| Links |
PubMed Online version:10.1006/jmbi.1993.1264 |
| Keywords |
Amino Acid Sequence; Chromatography, Affinity; Chromatography, Ion Exchange; Crystallization; Escherichia coli/enzymology; Escherichia coli/genetics; Fumarate Hydratase/chemistry; Fumarate Hydratase/genetics; Fumarate Hydratase/isolation & purification; Genes, Bacterial; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/isolation & purification; Kinetics; Molecular Sequence Data; Protein Conformation; X-Ray Diffraction |
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