PMID:8486276
| Citation |
Bentley, J, Hyatt, LS, Ainley, K, Parish, JH, Herbert, RB and White, GR (1993) Cloning and sequence analysis of an Escherichia coli gene conferring bicyclomycin resistance. Gene 127:117-20 |
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| Abstract |
We have cloned and sequenced DNA from Escherichia coli that, when present in a high-copy-number plasmid, confers resistance to the diketopiperazine antibiotic, bicyclomycin (Bc). The DNA includes a 378-amino-acid open reading frame (ORF), disruption of which results in the loss of Bc resistance. This ORF contains the BcR gene. Studies using the minicell expression system reveal that a polypeptide of 31 kDa is produced from this cloned region. The ORF maps at 47.1 min on the E. coli genome map. Sequence comparison between the translated ORF and a protein database reveal between 26.5 and 23.4% aa sequence homology to bacterial transmembrane (TM) proteins including those mediating chloramphenicol (Cm) and tetracycline (Tc) resistance and an arabinose-proton symport protein. Sequence analysis using the Diagon program showed the BcR gene product (BcR) had homology with the N-terminal regions of the CmR and TcR-encoded proteins and weak N-terminal homology with the arabinose-proton symport protein. Hydropathy profiles of the BcR protein and CmR products show a striking similarity, both having twelve predicted TM domains. |
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| Keywords |
Amino Acid Sequence; Anti-Bacterial Agents/pharmacology; Base Sequence; Bicyclo Compounds/pharmacology; Bicyclo Compounds, Heterocyclic; Cloning, Molecular; DNA, Bacterial; Drug Resistance, Microbial/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/drug effects; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Open Reading Frames; Restriction Mapping; Sequence Homology, Amino Acid |
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