PMID:8464075

Citation	Rodseth, L and Quiocho, FA (1993) Crystallization of the maltodextrin-binding protein for active transport and chemotaxis in several different liganded and mutant forms. J. Mol. Biol. 230:675-8
Abstract	We have crystallized the maltodextrin-binding protein complexed with two cyclic maltodextrins, and with no ligand at all. We have also grown crystals of two complexes of a mutant of maltodextrin-binding protein whose transport specificity has been altered. Studies of these crystals should provide considerable information about protein-oligosaccharide interactions, the effects of these interactions on oligosaccharide conformation, and particularly about the function of the periplasmic receptors in transport and chemotaxis.
Links	PubMed Online version:10.1006/jmbi.1993.1182
Keywords	Bacterial Proteins/chemistry; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Biological Transport, Active; Carbohydrate Conformation; Carrier Proteins/chemistry; Carrier Proteins/isolation & purification; Carrier Proteins/metabolism; Chemotaxis; Crystallization; Escherichia coli/metabolism; Escherichia coli Proteins; Oligosaccharides/chemistry; Oligosaccharides/metabolism; Protein Binding; Protein Conformation; Recombinant Proteins/chemistry
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