PMID:8462874

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Citation

Henrissat, B (1993) Hidden domains and active site residues in beta-glycanase-encoding gene sequences? Gene 125:199-204

Abstract

Reading-frame corrective shifts in the nucleotide sequence upstream, within, or downstream from the putative coding region of several beta-glycanase-encoding genes reported in the literature reveal hidden active-site residues or even additional domains, including a cellulose-binding domain on a beta-mannanase-encoding gene. These findings also help in assigning, to cellulase family A, two enzymes previously found to lack sequence similarity with known cellulase families.

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Keywords

Amino Acid Sequence; Bacteria/enzymology; Bacteria/genetics; Base Sequence; Binding Sites; Computer Simulation; DNA, Bacterial; Glycoside Hydrolases/genetics; Glycoside Hydrolases/metabolism; Molecular Sequence Data; Open Reading Frames; Sequence Homology, Amino Acid

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