PMID:8437561
| Citation |
Ueguchi, C, Kakeda, M and Mizuno, T (1993) Autoregulatory expression of the Escherichia coli hns gene encoding a nucleoid protein: H-NS functions as a repressor of its own transcription. Mol. Gen. Genet. 236:171-8 |
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| Abstract |
The Escherichia coli nucleoid protein, H-NS (or H1a), appears to influence the regulation of a variety of unrelated E. coli genes and operons. To gain an insight into the regulation of the hns gene itself, we constructed in this study a hns-lacZ transcriptional fusion gene and inserted a single copy at the att lambda locus on the E. coli chromosome. Expression of hns transcription appeared to be moderately regulated in a growth phase-dependent manner. It also emerged that hns transcription is under negative autoregulation, at least in the logarithmic growth phase. The results of in vitro transcription experiments confirmed that H-NS functions as a repressor for its own transcription. Thus, H-NS was shown to exhibit relatively high affinity for the DNA sequence encompassing the hns promoter region, as compared with a non-specific sequence. These results support the view that the nucleoid protein, H-NS, can function as a transcriptional regulator. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Proteins; Cell Division; Cell-Free System; DNA, Bacterial/metabolism; DNA, Recombinant/genetics; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial/genetics; Homeostasis; Lac Operon/genetics; Nuclear Proteins; Promoter Regions, Genetic/genetics; Repressor Proteins/genetics; Transcription, Genetic |
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