PMID:8432722
| Citation |
Meinnel, T, Guillon, JM, Mechulam, Y and Blanquet, S (1993) The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control. J. Bacteriol. 175:993-1000 |
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| Abstract |
The genetic organization near the recently cloned fmt gene, encoding Escherichia coli methionyl-tRNA(fMet) formyltransferase (J. M. Guillon, Y. Mechulam, J. M. Schmitter, S. Blanquet, and G. Fayat, J. Bacteriol. 174:4294-4301, 1992), has been studied. The fmt gene, which starts at a GUG codon, is cotranscribed with another gene, fms, and the transcription start site of this operon has been precisely mapped. Moreover, the nucleotide sequence of a 1,379-bp fragment upstream from fmt reveals two additional open reading frames, in the opposite polarity. In the range of 0.3 to 2 doublings per h, the intracellular methionyl-tRNA(fMet) formyltransferase concentration remains constant, providing, to our knowledge, the first example of a gene component of the protein synthesis apparatus escaping metabolic control. When the gene fusion technique was used for probing, no effect on fmt expression of the concentrations of methionyl-tRNA(fMet) formyltransferase or tRNA(fMet) could be found. The possibility that the fmt gene, the product of which is present in excess to ensure full N acylation of methionyl-tRNA(fMet), could be expressed in a constitutive manner is discussed. |
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| Keywords |
Acyltransferases/genetics; Amino Acid Sequence; Base Sequence; Codon; DNA, Bacterial/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Hydroxymethyl and Formyl Transferases; Molecular Sequence Data; N-Formylmethionine/metabolism; Oligodeoxyribonucleotides/chemistry; Peptide Chain Initiation, Translational; RNA, Transfer, Amino Acyl/metabolism; RNA, Transfer, Met |
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