PMID:8432709
| Citation |
Choe, M and Reznikoff, WS (1993) Identification of the regulatory sequence of anaerobically expressed locus aeg-46.5. J. Bacteriol. 175:1165-72 |
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| Abstract |
A newly identified anaerobically expressed locus, aeg-46.5, which is located at min 46.5 on Escherichia coli linkage map, was cloned and analyzed. The phenotype of this gene was studied by using a lacZ operon fusion. aeg-46.5 is induced anaerobically in the presence of nitrate in wild-type and narL cells. It is repressed by the narL gene product, as it showed derepressed anaerobic expression in narL mutant cells. We postulate that aeg-46.5 is subject to multiple regulatory systems, activation as a result of anaerobiosis, narL-independent nitrate-dependent activation, and narL-mediated repression. The regulatory region of aeg-46.5 was identified. A 304-bp DNA sequence which includes the regulatory elements was obtained, and the 5' end of aeg-46.5 mRNA was identified. It was verified that the anaerobic regulation of aeg-46.5 expression is controlled on the transcriptional level. Computer analysis predicted possible control sites for the NarL and FNR proteins. The proposed NarL site was found in a perfect-symmetry element. The aeg-46.5 regulatory elements are adjacent to, but divergent from, those of the eco gene. |
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| Keywords |
Amino Acid Sequence; Anaerobiosis; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Molecular Sequence Data; RNA, Messenger/genetics; Regulatory Sequences, Nucleic Acid; Repressor Proteins/genetics; Sequence Deletion; Transcription, Genetic |
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