PMID:8423162
| Citation |
Krishnan, BR and Berg, DE (1993) Viability of folA-null derivatives of wild-type (thyA+) Escherichia coli K-12. J. Bacteriol. 175:909-11 |
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| Abstract |
Dihydrofolate reductase (the folA gene product) catalyzes the synthesis of tetrahydrofolate, a key methyl donor in many biosynthetic pathways. Loss of folA had been thought to be lethal to wild-type (thyA+) Escherichia coli. Viable folA-null derivatives of thyA+ E. coli were obtained, however, by recombining a folA deletion linked to a Kanr selectable marker into a lambda folA+ phage and using this phage to transduce cells to kanamycin resistance. folA-null strains were slow growing, formed small colonies, and were auxotrophic for thymidine, adenine, methionine, glycine, and pantothenate. |
| Links | |
| Keywords |
Base Sequence; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Deletion; Genes, Bacterial; Molecular Sequence Data; Oligodeoxyribonucleotides/chemistry; Polymerase Chain Reaction; Restriction Mapping; Tetrahydrofolate Dehydrogenase/genetics |
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