PMID:8419307
Citation |
Yamada, M, Asaoka, S, Saier, MH Jr and Yamada, Y (1993) Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression. J. Bacteriol. 175:568-71 |
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Abstract |
DNA sequence and expressional analyses of the gcd gene of Escherichia coli K-12 W3110 revealed that two promoters that were detected were regulated negatively by cyclic AMP and positively by oxygen. Sequence conservation of the gcd gene between E. coli K-12 W3110 and PPA42 suggests that glucose dehydrogenase is required for the E. coli cells, even though it ordinarily exists as an apoprotein. |
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Keywords |
Amino Acid Sequence; Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Genotype; Glucose 1-Dehydrogenase; Glucose Dehydrogenases/genetics; Glucose Dehydrogenases/metabolism; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Transcription, Genetic |
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