PMID:8416909
| Citation |
Damerau, K and St John, AC (1993) Role of Clp protease subunits in degradation of carbon starvation proteins in Escherichia coli. J. Bacteriol. 175:53-63 |
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| Abstract |
When deprived of a carbon source, Escherichia coli induces the synthesis of a group of carbon starvation proteins. The degradation of proteins labeled during starvation was found to be an energy-dependent process which was inhibited by the addition of KCN and accelerated when cells were resupplied with a carbon source. The degradation of the starvation proteins did not require the ATP-dependent Lon protease or the energy-independent proteases protease I, protease IV, OmpT, and DegP. During starvation, mutants lacking either the ClpA or ClpP subunit of the ATP-dependent Clp protease showed a partial reduction in the degradation of starvation proteins. Strains lacking ClpP failed to increase degradation of starvation proteins when glucose was added to starving cells. The clpP mutants showed a competitive disadvantage compared with wild-type cells when exposed to repeated cycles of carbon starvation and growth. Surprisingly, the glucose-stimulated, ClpP-dependent degradation of starvation proteins did not require either the ClpA or ClpB protein. The patterns of synthesis of starvation proteins were similar in clpP+ and clpP cells. The clpP mutants had reduced rates of degradation of certain starvation proteins in the membrane fraction when a carbon source was resupplied to the starved cells. |
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| Keywords |
ATP-Dependent Proteases; Adenosine Triphosphate/metabolism; Bacterial Proteins/metabolism; Chloramphenicol/pharmacology; Energy Metabolism; Enzyme Induction; Escherichia coli/drug effects; Escherichia coli/metabolism; Escherichia coli Proteins; Glucose/deficiency; Glucose/metabolism; Heat-Shock Proteins; Membrane Proteins/metabolism; Potassium Cyanide/pharmacology; Protease La; Selection, Genetic; Serine Endopeptidases/metabolism; Time Factors |
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