PMID:8412702

Citation	Mudd, EA and Higgins, CF (1993) Escherichia coli endoribonuclease RNase E: autoregulation of expression and site-specific cleavage of mRNA. Mol. Microbiol. 9:557-68
Abstract	Mutations in the Escherichia coli rne (ams) gene have a general effect on the rate of mRNA decay in vivo. Using antibodies we have shown that the product of the rne gene is a polypeptide of relative mobility 180 kDa. However, proteolytic fragments as small as 70 kDa, which can arise during purification, also exhibit RNase E activity. In vitro studies demonstrate that the rne gene product, RNase E, is an endoribonuclease that cleaves mRNA at specific sites. RNase E cleaves rne mRNA and autoregulates the expression of the rne gene. In addition we demonstrate RNase E-dependent endonucleolytic cleavage of ompA mRNA, at a site known to be rate-determining for degradation and reported to be cleaved by RNase K. Our data are consistent with RNase K being a proteolytic fragment of RNase E.
Links	PubMed
Keywords	Antibodies, Bacterial; Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Outer Membrane Proteins/genetics; Base Sequence; Endoribonucleases/biosynthesis; Endoribonucleases/genetics; Endoribonucleases/immunology; Endoribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional; Recombinant Proteins/biosynthesis; Substrate Specificity
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