PMID:8405944
| Citation |
Bebbington, KJ and Williams, HD (1993) Investigation of the role of the cydD gene product in production of a functional cytochrome d oxidase in Escherichia coli. FEMS Microbiol. Lett. 112:19-24 |
|---|---|
| Abstract |
The cydD gene is needed for the formation of a functional cytochrome d oxidase in the aerobic respiratory chain of Escherichia coli. In this paper we demonstrate that transcription from a cydA-lacZ gene fusion is not significantly affected in a cydD mutant. This, together with the finding that subunit I of cytochrome d is present in cytoplasmic membranes of a cydD mutant, rules out a role for CydD in the regulation of cytochrome d expression or the assembly of its polypeptides into the membrane. The activity of the haem d-containing catalase HP II was found to be similar in a cydD mutant and the wild-type. Therefore, if CydD has a role in haem d biosynthesis it must be in a unique step only associated with the synthesis of the haem d prosthetic group of cytochrome d. |
| Links | |
| Keywords |
Catalase/genetics; Catalase/metabolism; Cloning, Molecular; Cytochrome d Group/biosynthesis; Cytochrome d Group/genetics; Cytochromes/biosynthesis; Cytochromes/genetics; Electron Transport Chain Complex Proteins; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Heme/biosynthesis; Lac Operon; Mutation; Oxidoreductases/biosynthesis; Oxidoreductases/genetics; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.