PMID:8405419
| Citation |
Menon, NK, Robbins, J, Der Vartanian, M, Patil, D, Peck, HD Jr, Menon, AL, Robson, RL and Przybyla, AE (1993) Carboxy-terminal processing of the large subunit of [NiFe] hydrogenases. FEBS Lett. 331:91-5 |
|---|---|
| Abstract |
Two electrophoretic forms of the large subunit of the soluble periplasmic [NiFe] hydrogenase from Desulfovibrio gigas have been detected by Western analysis. The faster moving form co-migrates with the large subunit from purified, active enzyme. Amino acid sequence and composition of the C-terminal tryptic peptide of the large subunit from purified hydrogenase revealed that it is 15 amino acids shorter than that predicted by the nucleotide sequence. Processing of the nascent large subunit occurs by C-terminal cleavage between His536 and Val537, residues which are highly conserved among [NiFe] hydrogenases. Mutagenesis of the analogous residues, His582 and Val583, in the E. coli hydrogenase-1 (HYD1) large subunit resulted in significant decrease in processing and HYD1 activity. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acids/analysis; Blotting, Western; Chromatography, Ion Exchange; Desulfovibrio/enzymology; Escherichia coli/enzymology; Hydrogenase/chemistry; Hydrogenase/genetics; Hydrogenase/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Processing, Post-Translational |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.