PMID:8396120
| Citation |
Moralejo, P, Egan, SM, Hidalgo, E and Aguilar, J (1993) Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli. J. Bacteriol. 175:5585-94 |
|---|---|
| Abstract |
The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to rhamnulose kinase for rhaB, a 400-amino-acid polypeptide corresponding to rhamnose isomerase for rhaA, and a 274-amino-acid polypeptide corresponding to rhamnulose-1-phosphate aldolase for rhaD. Transcriptional fusions of the three putative promoter regions to lacZ showed that only the rhaB leader region acted as a promoter, as indicated by the high beta-galactosidase activity induced by rhamnose, while no significant activity from the rhaA and rhaD constructions was detected. The rhaB transcription start site was mapped to -24 relative to the start of translation. Mutations in the catabolic genes were used to show that L-rhamnose may directly induce rhaBAD transcription. |
| Links | |
| Keywords |
Aldehyde-Lyases/genetics; Aldose-Ketose Isomerases; Amino Acid Sequence; Base Composition; Base Sequence; Carbohydrate Epimerases/genetics; Codon; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Multigene Family; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Restriction Mapping; Rhamnose/metabolism; Sequence Homology; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.