PMID:8395352

Citation	Allen, GC Jr, Dixon, NE and Kornberg, A (1993) Strand switching of a replicative DNA helicase promoted by the E. coli primosome. Cell 74:713-22
Abstract	The E. coli primosome assembles at an origin on a single-stranded DNA, like that of phi X174, to promote replication of that template. Upon conversion to the duplex form, the primosome can generate a rolling circle product from this template. Rolling circle synthesis implies the transfer of the DnaB helicase from its initial loading site on the viral strand to a displaced complementary strand. Isolated primosomes promote only unit-length synthesis; supplementation with PriC, DnaC, and DnaT is necessary to reconstitute rolling circle synthesis. Rolling circle replication is sensitive to salts, whereas primosome assembly and unit-length synthesis are not. Thus, the primosome promotes two distinct reactions: assembly for first-round synthesis and strand switching for rolling circle synthesis.
Links	PubMed
Keywords	Ammonium Sulfate/pharmacology; Bacterial Proteins; Bacteriophage phi X 174/metabolism; Chromosomes, Bacterial/physiology; DNA Helicases/metabolism; DNA Replication/drug effects; DNA, Single-Stranded/metabolism; DnaB Helicases; Escherichia coli/enzymology; Kinetics; Models, Structural; Time Factors
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