PMID:8388874
| Citation |
He, B, Choi, KY and Zalkin, H (1993) Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor. J. Bacteriol. 175:3598-606 |
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| Abstract |
A strategy was devised to identify Escherichia coli genes subject to coregulation by purR. From a data base search, similarities to the pur regulon cis-acting control site were found in 26 E. coli genes. Of five genes examined in which the putative pur operator is upstream of the coding sequence, glnB, prsA, and speA bound purified purine repressor in vitro. Binding of the repressor to a pur operator in these genes was dependent upon a corepressor. The pur operator in glnB is located between two major transcription start sites that were located by primer extension mapping. The effect of purR on expression of glnB, prsA, and speA was examined by using a lacZ reporter. The results indicated two- to threefold repression of these genes by purR. Coregulation by purR provides evidence that expands the pur regulon to include glnB, prsA, and speA. These genes have functions related to nucleotide metabolism. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial/genetics; Molecular Sequence Data; Operator Regions, Genetic/genetics; PII Nitrogen Regulatory Proteins; Polyamines/metabolism; Promoter Regions, Genetic/genetics; Recombinant Proteins/biosynthesis; Regulatory Sequences, Nucleic Acid/genetics; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ribose-Phosphate Pyrophosphokinase/genetics; Transcription, Genetic; beta-Galactosidase/biosynthesis; beta-Galactosidase/genetics |
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