PMID:8380170
| Citation |
Akiyama, M, Crooke, E and Kornberg, A (1993) An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon. J. Biol. Chem. 268:633-9 |
|---|---|
| Abstract |
A gene, ppx, that encodes a novel exopolyphosphatase of 513 amino acids (58,133 Da) was found downstream of the gene for polyphosphate kinase, ppk. Transcription of the ppx gene depends on the ppk promoters, indicating a polyphosphate (polyP) operon of ppk and ppx. Exopolyphosphatase, purified to homogeneity from overproducing cells, is judged to be a dimer of 58-kDa subunits. Orthophosphate is released processively from the ends of polyP approximately 500 residues long, but chains of approximately 15 residues compete poorly with polyP as substrate; ATP is not a substrate. Mg2+ (1 mM) and a high concentration of K+ (175 mM) support optimal activity. |
| Links | |
| Keywords |
Acid Anhydride Hydrolases; Amino Acid Sequence; Base Sequence; Chromatography, Ion Exchange; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Kinetics; Macromolecular Substances; Magnesium Chloride/pharmacology; Molecular Sequence Data; Operon; Phosphoric Monoester Hydrolases/genetics; Phosphoric Monoester Hydrolases/isolation & purification; Phosphoric Monoester Hydrolases/metabolism; Plasmids; Polyphosphates/metabolism; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Substrate Specificity; Thermodynamics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
