PMID:8366044
| Citation |
Carter, JR, Franden, MA, Aebersold, R and McHenry, CS (1993) Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme. J. Bacteriol. 175:5604-10 |
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| Abstract |
The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Polymerase III/biosynthesis; DNA Polymerase III/chemistry; DNA Polymerase III/genetics; DNA Polymerase III/isolation & purification; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Molecular Sequence Data; Open Reading Frames; Plasmids; Polymerase Chain Reaction |
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