PMID:8364098
| Citation |
Miczak, A and Apirion, D (1993) The rne gene and ribonuclease E. Biochimie 75:473-9 |
|---|---|
| Abstract |
The cloned rne+ gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. In vitro the RNA transcribed from the rne gene directs the synthesis of a number of polypeptides, one of which is identical in size to the in vivo product of the rne gene. A rabbit reticulocyte cell free extract programmed with this RNA produced RNase E activity. Thus, it is evident that the rne gene is the structural gene for RNase E. However, the in vivo product of the cloned RNase E gene is more thermolabile than the chromosomal gene product. When cells containing the rne plasmid were treated with chloramphenicol, the pre-existing RNase E became less heat labile with time. This leads to the suggestion that in the cell RNase E undergoes post-translational modification(s). |
| Links | |
| Keywords |
Animals; DNA, Bacterial/genetics; Endoribonucleases/genetics; Enzyme Stability; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Hot Temperature; Plasmids; Rabbits; Reticulocytes/metabolism; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.