PMID:8355660
| Citation |
Jones, KM, McPherson, MJ, Baron, AJ, Mattaj, IW, Riordan, CL and Wootton, JC (1993) The gdhA1 point mutation in Escherichia coli K12 CLR207 alters a key lysine residue of glutamate dehydrogenase. Mol. Gen. Genet. 240:286-9 |
|---|---|
| Abstract |
gdhA1 is a spontaneous mutant of Escherichia coli that causes complete loss of activity of the NADP-specific glutamate dehydrogenase (GDH) encoded by the gdhA gene. The gdhA1 mutational site has been identified by recombinational mapping, polymerase chain reaction (PCR) amplification and DNA sequencing, as an A to G transition at nucleotide 274 of the gdhA coding sequence, resulting in an amino acid change of lysine 92 to glutamic acid. The mutant enzyme forms hybrid hexamers with a wild-type GDH, providing a useful system for analysis of conformational integrity of mutational variants. |
| Links | |
| Keywords |
Base Sequence; Cloning, Molecular; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Glutamate Dehydrogenase/chemistry; Glutamate Dehydrogenase/genetics; Glutamate Dehydrogenase/metabolism; Lysine/genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Point Mutation; Polymerase Chain Reaction; Protein Conformation; Recombination, Genetic; Restriction Mapping |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.