PMID:8353127
| Citation |
Sprenger, GA (1993) Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria. Biochim. Biophys. Acta 1158:103-6 |
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| Abstract |
I have subcloned and sequenced the genes cmtB and cmtA of Escherichia coli K-12 which lie adjacent to the tkt gene on the chromosome. The genes cmtB and cmtA could encode a cytoplasmic protein (EIIA) and an integral-membrane protein (EIIBC), respectively, of the bacterial phospho enol pyruvate-dependent carbohydrate phosphotransferase system. High similarity to the Enzymes IIMtl of Escherichia coli K-12 (gene mtlA) and of Staphylococcus carnosus was detected, but the two genes did not complement mannitol-negative E. coli mutants without the use of a heterologous promoter. |
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| Keywords |
Amino Acid Sequence; Base Sequence; DNA, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Molecular Sequence Data; Monosaccharide Transport Proteins; Open Reading Frames; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Restriction Mapping; Sequence Homology, Amino Acid; Staphylococcus/enzymology; Staphylococcus/genetics; Transketolase/genetics |
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