PMID:8352802
| Citation |
Di Donato, A, Cafaro, V, de Nigris, M, Rizzo, M and D'Alessio, G (1993) The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region. Biochem. Biophys. Res. Commun. 194:1440-5 |
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| Abstract |
A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease. |
| Links | |
| Keywords |
Animals; Cattle; Protein Conformation; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/chemistry; Ribonuclease, Pancreatic/chemistry; Ribonuclease, Pancreatic/genetics; Ribonucleases/biosynthesis; Ribonucleases/chemistry; Ribonucleases/genetics; Semen/enzymology |
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