PMID:8343500
| Citation |
Kim, ST, Malhotra, K, Smith, CA, Taylor, JS and Sancar, A (1993) DNA photolyase repairs the trans-syn cyclobutane thymine dimer. Biochemistry 32:7065-8 |
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| Abstract |
DNA photolyases catalyze the splitting of the cyclobutane ring joining the two dihydropyrimidines of a pyrimidine dimer by a photoinduced electron-transfer reaction. Previous studies concluded that photolyase repairs only the cis-syn form of the eight stereoisomers of the cyclobutane pyrimidine dimer (Pyr[ ]Pyr). In this study we found that Escherichia coli photolyase binds to the trans-syn-I isomer of T[ ]T with about 10(4)-fold lower affinity than the cis-syn isomer but it repairs it relatively efficiently. |
| Links | |
| Keywords |
Base Sequence; DNA Repair; Deoxyribodipyrimidine Photo-Lyase/metabolism; Escherichia coli/enzymology; Isomerism; Molecular Sequence Data; Oligodeoxyribonucleotides; Photolysis; Pyrimidine Dimers/metabolism; Substrate Specificity; Ultraviolet Rays |
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