PMID:8341603
| Citation |
Carter, JR, Franden, MA, Aebersold, R, Kim, DR and McHenry, CS (1993) Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme. Nucleic Acids Res. 21:3281-6 |
|---|---|
| Abstract |
The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon; DNA Polymerase III/genetics; DNA Polymerase III/metabolism; DNA Probes; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Amplification; Genes, Bacterial; Molecular Sequence Data; Sequence Homology, Amino Acid |
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