PMID:8335254
| Citation |
Kravetz, AN, Zakharova, MV, Beletskaya, IV, Sineva, EV, Denjmuchametov, MM, Petrov, SI, Glatman, LI and Solonin, AS (1993) The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and EcoHI. Gene 129:153-4 |
|---|---|
| Abstract |
The restriction endonucleases Eco1831I and EcoHI cleave before the first 5'-cytosine in the recognition sequence 5'-decreases CCSGG--3'/3'--GGSCC increases-5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine. |
| Links | |
| Keywords |
Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific/genetics; Deoxyribonucleases, Type II Site-Specific/metabolism; Escherichia coli/enzymology; Genes, Bacterial; Substrate Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.