PMID:8335254

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Citation

Kravetz, AN, Zakharova, MV, Beletskaya, IV, Sineva, EV, Denjmuchametov, MM, Petrov, SI, Glatman, LI and Solonin, AS (1993) The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and EcoHI. Gene 129:153-4

Abstract

The restriction endonucleases Eco1831I and EcoHI cleave before the first 5'-cytosine in the recognition sequence 5'-decreases CCSGG--3'/3'--GGSCC increases-5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.

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Keywords

Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific/genetics; Deoxyribonucleases, Type II Site-Specific/metabolism; Escherichia coli/enzymology; Genes, Bacterial; Substrate Specificity

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