PMID:8300537

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Citation

Figge, RM, Ramseier, TM and Saier, MH Jr (1994) The mannitol repressor (MtlR) of Escherichia coli. J. Bacteriol. 176:840-7

Abstract

The mannitol operon of Escherichia coli, encoding the mannitol-specific enzyme II of the phosphotransferase system (Mt1A) and mannitol phosphate dehydrogenase (Mt1D), is here shown to contain a single additional downstream open reading frame which encodes the mannitol repressor (Mt1R). Mt1R contains 195 amino acids and has a calculated molecular weight of 21,990 and a calculated pI of 4.5. It is homologous to the product of an open reading frame (URF2D) upstream of the E. coli gapB gene but represents a novel type of transcriptional regulatory protein.

Links

PubMed PMC205122

Keywords

Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Primers/chemistry; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Mannitol/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Operon; RNA, Messenger/genetics; Repressor Proteins/genetics; Sequence Alignment; Sequence Homology, Amino Acid; Transcription, Genetic

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