PMID:8277941
| Citation |
Liebl, W, Gabelsberger, J and Schleifer, KH (1994) Comparative amino acid sequence analysis of Thermotoga maritima beta-glucosidase (BglA) deduced from the nucleotide sequence of the gene indicates distant relationship between beta-glucosidases of the BGA family and other families of beta-1,4-glycosyl hydrolases. Mol. Gen. Genet. 242:111-5 |
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| Abstract |
The primary structure of the bglA gene region encoding a beta-glucosidase of Thermotoga maritima strain MSB8 was determined. The bglA gene has the potential to code for a polypeptide of 446 amino acids with a predicted molecular mass of 51,545 Da. The T. maritima beta-glucosidase (BglA) was overexpressed in E. coli at a level comprising approximately 15-20% of soluble cellular protein. Based on its amino acid sequence, as deduced from the nucleotide sequence of the gene, BglA can be classified as a broad-specificity beta-glucosidase and as a member of the beta-glucosidase family BGA, in agreement with the results of enzymatic characterization of the recombinant protein. Comparative sequence analysis revealed distant amino acid sequence similarities between BGA family beta-glucosidases, a beta-xylosidase, beta-1,4-glycanases of the enzyme family F (mostly xylanases), and other families of beta-1,4-glycosyl hydrolases. This result indicates that BGA beta-glucosidases may comprise one enzyme family within a large 'enzyme order' of retaining beta-glycosyl hydrolases, and that the members of these enzyme groups may be inter-related at the level of active site architecture and perhaps even on the level of overall three-dimensional fold. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Base Composition; Base Sequence; Cloning, Molecular; Consensus Sequence; Conserved Sequence; Escherichia coli/enzymology; Glucuronidase/chemistry; Glucuronidase/classification; Glycoside Hydrolases/chemistry; Glycoside Hydrolases/classification; Gram-Negative Anaerobic Bacteria/enzymology; Molecular Sequence Data; Open Reading Frames; Protein Conformation; Recombinant Fusion Proteins; Sequence Alignment; Substrate Specificity; Xylosidases/chemistry; Xylosidases/classification; beta-Glucosidase/chemistry; beta-Glucosidase/classification; beta-Glucosidase/genetics |
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