PMID:8262918
| Citation |
Tate, CG and Henderson, PJ (1993) Membrane topology of the L-rhamnose-H+ transport protein (RhaT) from enterobacteria. J. Biol. Chem. 268:26850-7 |
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| Abstract |
The L-rhamnose-H+ symporter (RhaT) is a 344-amino acid integral membrane protein, found in many Enterobacteria, which couples the uptake of the sugar L-rhamnose with the inward movement of protons. Based on its hydropathy profile and the application of von Heijne's "positive inside" rule (von Heijne, G. (1992) J. Mol. Biol. 225, 487-494), a model of the L-rhamnose-H+ symport protein (RhaT) is proposed containing 10 transmembrane helices with the NH2 and COOH termini in the periplasm. This model was tested by the creation of random beta-lactamase (Bla) fusions. The data from 33 unique, randomly generated, RhaT-Bla fusions and from 5 site-specific fusions supported the proposed topology between transmembrane helices 2-10. However, the localization of the putative first hydrophilic loop and the NH2 terminus was not possible because the beta-lactamase fusions in this region were shown to be unreliable indicators of the topology of RhaT. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Base Sequence; Blotting, Western; Escherichia coli/chemistry; Escherichia coli Proteins; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/genetics; Molecular Sequence Data; Protein Conformation; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Rhamnose; Salmonella typhimurium/chemistry; Symporters; beta-Lactamases/chemistry; beta-Lactamases/genetics |
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