PMID:8260940

Citation	Takahashi, K, Hattori, T, Shindo, H, Noji, S, Nohno, T and Taniguchi, S (1993) Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by 31P-NMR spectroscopy. Biochem. Mol. Biol. Int. 31:161-8
Abstract	The sequential transphosphorylation from autophosphorylated nitrate-sensing protein (NarX) to the transcriptional regulator protein (NarL), both operating in signal transduction to control the expression of the respiratory nitrate reductase (nar) operon in E. coli, was demonstrated with an in vitro reconstructed system to function similarly to other bacterial two-component regulatory systems. Over-expression system established by means of the pT7 promoter/polymerase provided both NarX and NarL proteins to reconstruct the in vitro transphosphorylation system. The phosphorylated NarL was detected, and the unstable phosphorylated group was directly assigned to acyl phosphate in the in vitro system by 31P-NMR spectroscopy.
Links	PubMed
Keywords	Autoradiography; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Escherichia coli Proteins; Magnetic Resonance Spectroscopy; Operon; Phosphorylation; Protein Kinases; Transcription, Genetic
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