PMID:8243665
| Citation |
Spencer, JB, Stolowich, NJ, Roessner, CA and Scott, AI (1993) The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase. FEBS Lett. 335:57-60 |
|---|---|
| Abstract |
Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III to produce precorrin-2, hence it was given the trivial name uro'gen III methylase. We now report that in addition to methylase activity, the CysG protein catalyses both the NAD+ dependent oxidation of precorrin-2 to sirohydrochlorin, but also the insertion of iron into this oxidized intermediate, thereby producing siroheme. Thus CysG is a multifunctional protein solely responsible for siroheme synthesis from uro'gen III in E. coli, and accordingly is renamed siroheme synthase. |
| Links | |
| Keywords |
Amino Acid Sequence; Binding Sites; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Heme/analogs & derivatives; Heme/biosynthesis; Magnetic Resonance Spectroscopy; Methylation; Methyltransferases/chemistry; Methyltransferases/genetics; Methyltransferases/metabolism; Molecular Sequence Data; Multienzyme Complexes/chemistry; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; NAD/metabolism; NAD/pharmacology; Sequence Homology, Amino Acid; Uroporphyrinogens/metabolism |
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