PMID:8241274
| Citation |
Sprenger, GA (1993) Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene. Biochim. Biophys. Acta 1216:307-10 |
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| Abstract |
The gene tkt for the enzyme transketolase (TK; EC 2.2.1.1) of Escherichia coli K-12 was subcloned and the complete DNA sequence was determined from both strands. An open reading frame with 1992 bp was detected that could encode a protein with a subunit mass of 72,143. The gene forms a monocistronic operon and is transcribed counterclockwise to the E. coli chromosome. From recombinant strains, the enzyme was purified and 39 N-terminal amino acid residues were determined. The DNA-derived protein sequence showed 49.5% identical amino acid residues with the transketolase of Rhodobacter sphaeroides, 46.8% identity with the enzyme from Saccharomyces cerevisiae, and 28.9% identity with the human transketolase. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Conserved Sequence; DNA, Bacterial/biosynthesis; DNA, Bacterial/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Sequence Data; Open Reading Frames; Restriction Mapping; Sequence Alignment; Transketolase/genetics |
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