PMID:8226656
| Citation |
Neal, BL, Brown, PK and Reeves, PR (1993) Use of Salmonella phage P22 for transduction in Escherichia coli. J. Bacteriol. 175:7115-8 |
|---|---|
| Abstract |
A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera. |
| Links | |
| Keywords |
Bacteriophage P22/genetics; Bacteriophage P22/metabolism; Cloning, Molecular/methods; Cosmids; Drug Resistance, Microbial/genetics; Escherichia coli/genetics; Genes, Bacterial; Multigene Family; Plasmids; R Factors; Restriction Mapping; Salmonella/genetics; Transduction, Genetic |
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